P-291 Seeking Arrangements: A New Cleavage-Stage Biomarker from 3D Morphokinetics

Abstract Study question What can we learn from 3D reconstructions of cleavage-stage embryos derived Hoffman modulation contrast (HMC) time-lapses? Summary answer A simple spatial biomarker extracted embryo at the t4 and t8 stages is associated with blastulation, blastocyst quality, pregnancy live birth. known already Several works have demonstrated significant associations between cell arrangement blastulation potential. However, no studies investigated impacts beyond stage in a clinical setting owing to difficulties visualising structure safe, cost-effective manner. In previous ESHRE meeting, He et al. presented deep learning system for reconstruction HMC focal stacks recorded standard timelapse incubators. this work, use aforementioned understand networks present investigate their outcomes. design, size, duration The study was retrospective analysis two imaging datasets different clinics. first dataset (DS1) consisted 162 information on Gardner grade. second (DS2) 202 grade, pregnancy, birth PGT-A. All data captured 11 planes Embryoscope incubators 2018 2020. Participants/materials, setting, methods proposed by used reconstruct stacks. Networks contacts were resulting models each embryo’s mean per computed. Statistical average respect outcomes carried out using unpaired t-tests. Moreover, contact compared identify similar arrangements. Main results role chance At t4, higher number greater rates DS1 (2.59 vs 2.36, blastulated non-blastulated, p = 0.029) quality both 2.37, good poor, 0.010) DS2 (2.51 2.35, 0.017) where ‘good’ defined as having an embryologist-provided grade EXP>2, ICM>C TE>C. t8, increased (3.36 3.09, 0.017), (3.32 2.87, pregnant not pregnant, 0.003) (3.40 2.90, birth, 0.0003). No found miscarriage or aneuploidy. correlated those (r 0.15, 95% CI [-0.043, 0.34]). While 4-cell fell neatly into 9 distinct arrangements 5 most common (tetrahedral, pseudotetrahedral, planar, closed-Y linear) accounting embryos, 8-cell displayed great degree variation 59 arrangements, largest such group representing only 8 embryos. Limitations, reasons caution small. subject survivorship bias it contained PGT-A which necessarily entailed successful blastulation. Furthermore, applicable all especially obscured well undergone compaction. Wider implications findings This work provides evidence relevance human preimplantation stage, may improve selection techniques D3 transfers. our strong case further investigation biomarkers morphokinetics. Trial registration N/A